Clinical Proteomics:Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT .
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Biotin-PEG4-Alkyne Click Chemistry Tools Cat# TA105 6-Azido-L-lysine-HCl Jena Bioscience Cat# CLK-AA009 Trizma hydrochloride solution Sigma-Aldrich Cat# T2194 Sodium dodecyl sulfate Sigma-Aldrich Cat# 436143 Triton X-100 Sigma-Aldrich Cat# 11332481001 Bovine Serum Albumin Sigma-Aldrich Cat# 2153 Dimethyl sulfoxide Sigma-Aldrich Cat# D8418 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 PhosSTOP Sigma-Aldrich Cat# 04906837001 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 (Continued on next page) 16 Cell Reports 43, 113881, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S solution Sigma-Aldrich Cat# P7170 NuPAGE MOPS SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0001 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0002 NuPAGE Transfer Buffer (20X) Thermo Fisher Scientific Cat# NP00061 Gibco PBS, pH 7.4 Thermo Fisher Scientific Cat# 10010023 UltraPure Dnase/Rnase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 TRIzol Reagent Thermo Fisher Scientific Cat# 15596018 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Dynabeads MyOne Streptavidin C1 Thermo Fisher Scientific Cat# 65001 LY3214996 Cayman Chemicals Cat#27936 recombinant Plasminogen protein abcam Cat#200264 recombinant Plasmin protein abcam Cat#90928 Critical commercial assays RC DC Protein Assay Kit II Bio-Rad Cat# 5000122 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368813 Click-&-Go Protein Reaction Buffer Kit Click Chemistry Tools Cat# 1262 Plasmin Activity Assay Kit (Fluorometric) abcam Cat# ab204728 Plasminogen ELISA (mouse) abcam Cat# ab198511 Plasminogen ELISA (human) abcam Cat# ab108893 PAI-1 (SERPINE1) (mouse) abcam Cat# ab197752 PAI-1 (SERPINE1) (human) abcam Cat# ab269373 uPA (mouse) abcam Cat# ab245727 tPA (mouse) abcam Cat# ab233615 Deposited data Proteomics This paper ProteomeXchange with identifier PXD043644 CALERIETM Muscle RNAseq Das et al.41 https://doi.org/10.1111/ acel.13963 Experimental models: Organisms/strains Mouse: C57BL6/J Jackson Laboratories Cat# 000664 Mouse: C57BL/ 6-Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm/J Jackson Laboratories Cat# 028071 Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J Jackson Laboratories Cat# 003574 Mouse: B6.FVB-Tg(Adipoq-cre)1Evdr/J Jackson Laboratories Cat# 028020 Mouse: B6.Cg-Tg(ACTA1-cre)79Jme/J Jackson Laboratories Cat# 006149 Oligonucleotides See Table S2 This paper N/A Recombinant DNA AAV8-U6-PLG-shRNA Vector Biosystems Cat# shAAV-268877 AAV8-GFP-U6-shRNA Vector Biosystems Custume order Software and algorithms R(v4.0.5) R Core Team https://www.r-project.org/ RStudio(v1.4.1106) RStudio https://www.rstudio.com/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ ImageJ ImageJ https://ImageJ.nih.gov/ij FlowJo FlowJo https://www.flojo.com/ Cell Reports 43, 113881, March 26, 2024 17
Recombinant:Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT .
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Biotin-PEG4-Alkyne Click Chemistry Tools Cat# TA105 6-Azido-L-lysine-HCl Jena Bioscience Cat# CLK-AA009 Trizma hydrochloride solution Sigma-Aldrich Cat# T2194 Sodium dodecyl sulfate Sigma-Aldrich Cat# 436143 Triton X-100 Sigma-Aldrich Cat# 11332481001 Bovine Serum Albumin Sigma-Aldrich Cat# 2153 Dimethyl sulfoxide Sigma-Aldrich Cat# D8418 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 PhosSTOP Sigma-Aldrich Cat# 04906837001 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 (Continued on next page) 16 Cell Reports 43, 113881, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S solution Sigma-Aldrich Cat# P7170 NuPAGE MOPS SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0001 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0002 NuPAGE Transfer Buffer (20X) Thermo Fisher Scientific Cat# NP00061 Gibco PBS, pH 7.4 Thermo Fisher Scientific Cat# 10010023 UltraPure Dnase/Rnase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 TRIzol Reagent Thermo Fisher Scientific Cat# 15596018 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Dynabeads MyOne Streptavidin C1 Thermo Fisher Scientific Cat# 65001 LY3214996 Cayman Chemicals Cat#27936 recombinant Plasminogen protein abcam Cat#200264 recombinant Plasmin protein abcam Cat#90928 Critical commercial assays RC DC Protein Assay Kit II Bio-Rad Cat# 5000122 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368813 Click-&-Go Protein Reaction Buffer Kit Click Chemistry Tools Cat# 1262 Plasmin Activity Assay Kit (Fluorometric) abcam Cat# ab204728 Plasminogen ELISA (mouse) abcam Cat# ab198511 Plasminogen ELISA (human) abcam Cat# ab108893 PAI-1 (SERPINE1) (mouse) abcam Cat# ab197752 PAI-1 (SERPINE1) (human) abcam Cat# ab269373 uPA (mouse) abcam Cat# ab245727 tPA (mouse) abcam Cat# ab233615 Deposited data Proteomics This paper ProteomeXchange with identifier PXD043644 CALERIETM Muscle RNAseq Das et al.41 https://doi.org/10.1111/ acel.13963 Experimental models: Organisms/strains Mouse: C57BL6/J Jackson Laboratories Cat# 000664 Mouse: C57BL/ 6-Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm/J Jackson Laboratories Cat# 028071 Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J Jackson Laboratories Cat# 003574 Mouse: B6.FVB-Tg(Adipoq-cre)1Evdr/J Jackson Laboratories Cat# 028020 Mouse: B6.Cg-Tg(ACTA1-cre)79Jme/J Jackson Laboratories Cat# 006149 Oligonucleotides See Table S2 This paper N/A Recombinant DNA AAV8-U6-PLG-shRNA Vector Biosystems Cat# shAAV-268877 AAV8-GFP-U6-shRNA Vector Biosystems Custume order Software and algorithms R(v4.0.5) R Core Team https://www.r-project.org/ RStudio(v1.4.1106) RStudio https://www.rstudio.com/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ ImageJ ImageJ https://ImageJ.nih.gov/ij FlowJo FlowJo https://www.flojo.com/ Cell Reports 43, 113881, March 26, 2024 17
Whole Genome Amplification:Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT .
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Biotin-PEG4-Alkyne Click Chemistry Tools Cat# TA105 6-Azido-L-lysine-HCl Jena Bioscience Cat# CLK-AA009 Trizma hydrochloride solution Sigma-Aldrich Cat# T2194 Sodium dodecyl sulfate Sigma-Aldrich Cat# 436143 Triton X-100 Sigma-Aldrich Cat# 11332481001 Bovine Serum Albumin Sigma-Aldrich Cat# 2153 Dimethyl sulfoxide Sigma-Aldrich Cat# D8418 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 PhosSTOP Sigma-Aldrich Cat# 04906837001 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 (Continued on next page) 16 Cell Reports 43, 113881, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S solution Sigma-Aldrich Cat# P7170 NuPAGE MOPS SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0001 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0002 NuPAGE Transfer Buffer (20X) Thermo Fisher Scientific Cat# NP00061 Gibco PBS, pH 7.4 Thermo Fisher Scientific Cat# 10010023 UltraPure Dnase/Rnase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 TRIzol Reagent Thermo Fisher Scientific Cat# 15596018 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Dynabeads MyOne Streptavidin C1 Thermo Fisher Scientific Cat# 65001 LY3214996 Cayman Chemicals Cat#27936 recombinant Plasminogen protein abcam Cat#200264 recombinant Plasmin protein abcam Cat#90928 Critical commercial assays RC DC Protein Assay Kit II Bio-Rad Cat# 5000122 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368813 Click-&-Go Protein Reaction Buffer Kit Click Chemistry Tools Cat# 1262 Plasmin Activity Assay Kit (Fluorometric) abcam Cat# ab204728 Plasminogen ELISA (mouse) abcam Cat# ab198511 Plasminogen ELISA (human) abcam Cat# ab108893 PAI-1 (SERPINE1) (mouse) abcam Cat# ab197752 PAI-1 (SERPINE1) (human) abcam Cat# ab269373 uPA (mouse) abcam Cat# ab245727 tPA (mouse) abcam Cat# ab233615 Deposited data Proteomics This paper ProteomeXchange with identifier PXD043644 CALERIETM Muscle RNAseq Das et al.41 https://doi.org/10.1111/ acel.13963 Experimental models: Organisms/strains Mouse: C57BL6/J Jackson Laboratories Cat# 000664 Mouse: C57BL/ 6-Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm/J Jackson Laboratories Cat# 028071 Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J Jackson Laboratories Cat# 003574 Mouse: B6.FVB-Tg(Adipoq-cre)1Evdr/J Jackson Laboratories Cat# 028020 Mouse: B6.Cg-Tg(ACTA1-cre)79Jme/J Jackson Laboratories Cat# 006149 Oligonucleotides See Table S2 This paper N/A Recombinant DNA AAV8-U6-PLG-shRNA Vector Biosystems Cat# shAAV-268877 AAV8-GFP-U6-shRNA Vector Biosystems Custume order Software and algorithms R(v4.0.5) R Core Team https://www.r-project.org/ RStudio(v1.4.1106) RStudio https://www.rstudio.com/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ ImageJ ImageJ https://ImageJ.nih.gov/ij FlowJo FlowJo https://www.flojo.com/ Cell Reports 43, 113881, March 26, 2024 17
Protease Inhibitor:Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT .
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Biotin-PEG4-Alkyne Click Chemistry Tools Cat# TA105 6-Azido-L-lysine-HCl Jena Bioscience Cat# CLK-AA009 Trizma hydrochloride solution Sigma-Aldrich Cat# T2194 Sodium dodecyl sulfate Sigma-Aldrich Cat# 436143 Triton X-100 Sigma-Aldrich Cat# 11332481001 Bovine Serum Albumin Sigma-Aldrich Cat# 2153 Dimethyl sulfoxide Sigma-Aldrich Cat# D8418 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 PhosSTOP Sigma-Aldrich Cat# 04906837001 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 (Continued on next page) 16 Cell Reports 43, 113881, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S solution Sigma-Aldrich Cat# P7170 NuPAGE MOPS SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0001 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Scientific Cat# NP0002 NuPAGE Transfer Buffer (20X) Thermo Fisher Scientific Cat# NP00061 Gibco PBS, pH 7.4 Thermo Fisher Scientific Cat# 10010023 UltraPure Dnase/Rnase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 TRIzol Reagent Thermo Fisher Scientific Cat# 15596018 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Dynabeads MyOne Streptavidin C1 Thermo Fisher Scientific Cat# 65001 LY3214996 Cayman Chemicals Cat#27936 recombinant Plasminogen protein abcam Cat#200264 recombinant Plasmin protein abcam Cat#90928 Critical commercial assays RC DC Protein Assay Kit II Bio-Rad Cat# 5000122 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368813 Click-&-Go Protein Reaction Buffer Kit Click Chemistry Tools Cat# 1262 Plasmin Activity Assay Kit (Fluorometric) abcam Cat# ab204728 Plasminogen ELISA (mouse) abcam Cat# ab198511 Plasminogen ELISA (human) abcam Cat# ab108893 PAI-1 (SERPINE1) (mouse) abcam Cat# ab197752 PAI-1 (SERPINE1) (human) abcam Cat# ab269373 uPA (mouse) abcam Cat# ab245727 tPA (mouse) abcam Cat# ab233615 Deposited data Proteomics This paper ProteomeXchange with identifier PXD043644 CALERIETM Muscle RNAseq Das et al.41 https://doi.org/10.1111/ acel.13963 Experimental models: Organisms/strains Mouse: C57BL6/J Jackson Laboratories Cat# 000664 Mouse: C57BL/ 6-Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm/J Jackson Laboratories Cat# 028071 Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J Jackson Laboratories Cat# 003574 Mouse: B6.FVB-Tg(Adipoq-cre)1Evdr/J Jackson Laboratories Cat# 028020 Mouse: B6.Cg-Tg(ACTA1-cre)79Jme/J Jackson Laboratories Cat# 006149 Oligonucleotides See Table S2 This paper N/A Recombinant DNA AAV8-U6-PLG-shRNA Vector Biosystems Cat# shAAV-268877 AAV8-GFP-U6-shRNA Vector Biosystems Custume order Software and algorithms R(v4.0.5) R Core Team https://www.r-project.org/ RStudio(v1.4.1106) RStudio https://www.rstudio.com/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ ImageJ ImageJ https://ImageJ.nih.gov/ij FlowJo FlowJo https://www.flojo.com/ Cell Reports 43, 113881, March 26, 2024 17
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